e. histolytica ii elisa test kit  (TechLab Inc)

Journal: BMC Microbiology

Article Title: A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica , E. moshkovskii and E. dispar DNA in stool samples

doi: 10.1186/1471-2180-7-47

Figure Lengend Snippet: Comparison of results of nested multiplex PCR (Polymerase Chain Reaction), microscopy and culture on stool specimens.

Article Snippet: We are also grateful to TechLab, Inc. (Blacksburg, Va. USA) for providing the TechLab E. histolytica II ELISA test kit free of cost for the purpose of research.

Techniques: Multiplex Assay, Polymerase Chain Reaction, Microscopy, Infection

Journal: BMC Microbiology

Article Title: A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica , E. moshkovskii and E. dispar DNA in stool samples

doi: 10.1186/1471-2180-7-47

Figure Lengend Snippet: Sensitivity of PCR for detection of minimum number of E. moshkovskii (EM), E. histolytica (EH) and E. dispar (ED) cells. 0.05 gm of negative control stool specimen seeded with serially diluted Entamoeba cells corresponding to 10 6 cells (lane 1), 10 5 cells (lane 2), 10 4 cells (lane 3), 10 3 cells (lane 4), 10 2 cells (lane 5), and 10 cells (lane 6) were subjected to DNA extraction followed by PCR amplification. Amplified products were analyzed by agarose gel electrophoresis. The sizes of the amplification products are indicated on the left (in base pairs). Lanes 7, negative control (PCR without DNA)

Article Snippet: We are also grateful to TechLab, Inc. (Blacksburg, Va. USA) for providing the TechLab E. histolytica II ELISA test kit free of cost for the purpose of research.

Techniques: Negative Control, DNA Extraction, Amplification, Agarose Gel Electrophoresis

Journal: BMC Microbiology

Article Title: A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica , E. moshkovskii and E. dispar DNA in stool samples

doi: 10.1186/1471-2180-7-47

Figure Lengend Snippet: Detection of E. histolytica (A), E. dispar (B) and E. moshkovskii (C) in mixed cell lysates. To 1000 cells of E. dispar and E. moshkovskii (A) or E. histolytica and E. moshkovskii or (B), E. histolytica and E. dispar (C), 1000 cells (lane 1), 100 cells (lane 2), 10 cell (lane 3), 1 cell (lane 4), 0.1 cell (lane 5), 0.01 cell (lane 6) and 0.001 cell (lane 7) of the other species were added. Amplification was done by nested multiplex PCR.

Article Snippet: We are also grateful to TechLab, Inc. (Blacksburg, Va. USA) for providing the TechLab E. histolytica II ELISA test kit free of cost for the purpose of research.

Techniques: Amplification, Multiplex Assay

Journal: BMC Microbiology

Article Title: A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica , E. moshkovskii and E. dispar DNA in stool samples

doi: 10.1186/1471-2180-7-47

Figure Lengend Snippet: Differential detection of E. histolytica , E. moshkovskii and E. dispar by nested multiplex PCR on stool samples. The E. moshkovskii (EM), E. histolytica (EH) and E. dispar (ED) bands are 553, 439 and 174 bp, respectively. Lane-1, E. moshkovskii (mono infection); Lane-2, E. moshkovskii , E. histolytica and E. dispar (mixed infection); Lane-3, E. histolytica and E. dispar (mixed infection); Lane-4 E. moshkovskii and E. histolytica (mixed infection); Lane-5, E. moshkovskii and E. dispar (mixed infection); Lane-6 E. histolytica (mono infection), Lane-7 E. dispar (mono infection); Lane-8, 100 bp DNA ladder (Bangalore genei, Bangalore).

Article Snippet: We are also grateful to TechLab, Inc. (Blacksburg, Va. USA) for providing the TechLab E. histolytica II ELISA test kit free of cost for the purpose of research.

Techniques: Multiplex Assay, Infection

Journal: BMC Microbiology

Article Title: A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica , E. moshkovskii and E. dispar DNA in stool samples

doi: 10.1186/1471-2180-7-47

Figure Lengend Snippet: PCR positivity (%) of E. dispar , E. moshkovskii among the stool samples that were positive for E. histolytica/E. dispar / E. moshkovskii by microscopy and/or culture a .

Article Snippet: We are also grateful to TechLab, Inc. (Blacksburg, Va. USA) for providing the TechLab E. histolytica II ELISA test kit free of cost for the purpose of research.

Techniques: Microscopy

Journal: BMC Microbiology

Article Title: A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica , E. moshkovskii and E. dispar DNA in stool samples

doi: 10.1186/1471-2180-7-47

Figure Lengend Snippet: Comparison of results of nested multiplex PCR (Polymerase Chain Reaction) and TechLab E. histolytica II ELISA test performed on stool specimens.

Article Snippet: We are also grateful to TechLab, Inc. (Blacksburg, Va. USA) for providing the TechLab E. histolytica II ELISA test kit free of cost for the purpose of research.

Techniques: Multiplex Assay, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Infection

Journal: BMC Microbiology

Article Title: A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica , E. moshkovskii and E. dispar DNA in stool samples

doi: 10.1186/1471-2180-7-47

Figure Lengend Snippet: Primer sequences used for nested multiplex PCR

Article Snippet: We are also grateful to TechLab, Inc. (Blacksburg, Va. USA) for providing the TechLab E. histolytica II ELISA test kit free of cost for the purpose of research.

Techniques: Multiplex Assay